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minimum essential medium alpha media cat. #32561  (Thermo Fisher)


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    Thermo Fisher minimum essential medium alpha media cat. #32561
    Minimum Essential Medium Alpha Media Cat. #32561, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minimum essential medium alpha media cat. #32561/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    minimum essential medium alpha media cat. #32561 - by Bioz Stars, 2026-05
    90/100 stars

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    Overexpression of miR-20a activates osteogenic MC3T3-E1 cell differentiation. After transfecting the cells with miR-20a double-stranded miRNA mimics (50 or 100 nM), miRNA NC (50 or 100 nM), or transfection reagent only (control) for 12 h, the cultures of confluent MC3T3-E1 cells were placed <t>in</t> <t>α-MEM</t> medium with β-glycerophosphate (10 mM) and 50 µg/mL ascorbic acid. (A) qRT-PCR assays for ALP mRNA at 24 h. (B) Detection of ALP activity on day 3. (C) Determination of ECM mineralization by stained with Alizarin red S on day 14. Relative means and standard deviations were shown underneath (P<0.001; scale bar: 50 µm). Data are expressed as mean ± SD for n=3; **, P<0.01 vs. NC; ***, P<0.001 vs. NC. NC, negative control; qRT-PCR, real-time quantitative polymerase chain reaction; ALP, alkaline phosphatase; ECM, extracellular matrix.
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    Overexpression of miR-20a activates osteogenic MC3T3-E1 cell differentiation. After transfecting the cells with miR-20a double-stranded miRNA mimics (50 or 100 nM), miRNA NC (50 or 100 nM), or transfection reagent only (control) for 12 h, the cultures of confluent MC3T3-E1 cells were placed <t>in</t> <t>α-MEM</t> medium with β-glycerophosphate (10 mM) and 50 µg/mL ascorbic acid. (A) qRT-PCR assays for ALP mRNA at 24 h. (B) Detection of ALP activity on day 3. (C) Determination of ECM mineralization by stained with Alizarin red S on day 14. Relative means and standard deviations were shown underneath (P<0.001; scale bar: 50 µm). Data are expressed as mean ± SD for n=3; **, P<0.01 vs. NC; ***, P<0.001 vs. NC. NC, negative control; qRT-PCR, real-time quantitative polymerase chain reaction; ALP, alkaline phosphatase; ECM, extracellular matrix.
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    Overexpression of miR-20a activates osteogenic MC3T3-E1 cell differentiation. After transfecting the cells with miR-20a double-stranded miRNA mimics (50 or 100 nM), miRNA NC (50 or 100 nM), or transfection reagent only (control) for 12 h, the cultures of confluent MC3T3-E1 cells were placed in α-MEM medium with β-glycerophosphate (10 mM) and 50 µg/mL ascorbic acid. (A) qRT-PCR assays for ALP mRNA at 24 h. (B) Detection of ALP activity on day 3. (C) Determination of ECM mineralization by stained with Alizarin red S on day 14. Relative means and standard deviations were shown underneath (P<0.001; scale bar: 50 µm). Data are expressed as mean ± SD for n=3; **, P<0.01 vs. NC; ***, P<0.001 vs. NC. NC, negative control; qRT-PCR, real-time quantitative polymerase chain reaction; ALP, alkaline phosphatase; ECM, extracellular matrix.

    Journal: Annals of Translational Medicine

    Article Title: MiR-20a: a mechanosensitive microRNA that regulates fluid shear stress-mediated osteogenic differentiation via the BMP2 signaling pathway by targeting BAMBI and SMAD6

    doi: 10.21037/atm-22-2753

    Figure Lengend Snippet: Overexpression of miR-20a activates osteogenic MC3T3-E1 cell differentiation. After transfecting the cells with miR-20a double-stranded miRNA mimics (50 or 100 nM), miRNA NC (50 or 100 nM), or transfection reagent only (control) for 12 h, the cultures of confluent MC3T3-E1 cells were placed in α-MEM medium with β-glycerophosphate (10 mM) and 50 µg/mL ascorbic acid. (A) qRT-PCR assays for ALP mRNA at 24 h. (B) Detection of ALP activity on day 3. (C) Determination of ECM mineralization by stained with Alizarin red S on day 14. Relative means and standard deviations were shown underneath (P<0.001; scale bar: 50 µm). Data are expressed as mean ± SD for n=3; **, P<0.01 vs. NC; ***, P<0.001 vs. NC. NC, negative control; qRT-PCR, real-time quantitative polymerase chain reaction; ALP, alkaline phosphatase; ECM, extracellular matrix.

    Article Snippet: Cell culture Culturing of MC3T3-E1 cells, a murine pre-osteoblastic cell line (ATCC, Manassas, VA, USA) was performed in minimum essential medium alpha medium (α-MEM) media (Life Technologies, Grand Island, NY, USA) with 1% penicillin/streptomycin (Life Technologies) and fetal bovine serum (FBS; 10%, Life Technologies).

    Techniques: Over Expression, Cell Differentiation, Transfection, Control, Quantitative RT-PCR, Activity Assay, Staining, Negative Control, Real-time Polymerase Chain Reaction